The international cartagena protocol on biosafety deals primarily with the agricultural definition but many advocacy groups seek to expand it to include postgenetic threats. The maximum containment laboratory biosafety level 4. Comparison and analysis of biological agent category lists. Anyone working with any type of biological material must register with the biological safety program using the appropriate registration form and complete all appropriate training. Laboratory manual for the examirriltion of human semcn and sperm cervical mucus interaction. Who interim guidance on laboratory testing for coronavirus. The manual now covers risk assessment and safe use of recombinant dna technology, and provides guidelines for the comissioning and. This manual would be an excellent addition to the laboratory library. It is reported that 390 million people are infected annually, and about 96 million people among them have an apparent infection 1. For more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. Biological safety manual florida atlantic university. Manual for the laboratory diagnosis of measles and rubella virus infection, 3rd edition, june 2018 from the world health organization who this manual provides information about the who laboratory global network for measles and rubella including detailed protocols for collection and handling of specimens for confirmation of measles and rubella infections. Who laboratory manual for the examination and processing of. Since 1983, the world health organization who laboratory biosafety manual lbm has encouraged countries to implement basic concepts in biological safety and to develop.
Who laboratory biosafety manual lbm revision update. When centrifuging microbiological samples from the risk group ii according to the laboratory biosafety manual of the world health organization who, aerosoltight biological seals have to be used. Performing a risk assessment laboratory biosafety manual, 3rd edition, 2004 link to world health organization website guidelines for safe work practices in human and animal medical diagnostic laboratories link to cdc website. Since 1983, the world health organization who laboratory biosafety manual lbm has encouraged countries to implement basic concepts in biological safety and to develop national codes of practice for the safe. Centers for disease control and prevention cdc assessment tool for ebola treatment centers and assessment hospitals.
The world health organization who manual provides guidelines that enhance safety and security in containment laboratories if strictly adhered to. Good microbiological technique and appropriate use of biosafety equipment by well trained staff. Risk assessment and the classification of tb laboratories 2. Guidelines for environmental surveillance of poliovirus circulation. For this new edition, the manual has been extensively revised and expanded. As needed, an adhoc committee may be formed to consider policy implications for the university of emerging technologies and processes that have a biosafety component. Laboratory biosafety and biosecurity risk assessment. This specialized agency of the united nations published the first edition of its laboratory biosafety manual in 1983. World health organization laboratory biosafety manual, third edition. No or low individual and community risk a microorganism that is unlikely to cause human or animal disease. Health canada, health protection branch lab centre for disease control laboratory biosafety guidelines national academy press biosafety in the laboratory prudent practices for the handling and disposal of infectious materials world health organization laboratory biosafety manual module 4 mechanical, electrical, plumbing considerations i. The one most commonly referred to is the world health organizations laboratory safety manual world health organization, 2004a where an excellent compilation of the relationship between the risk groups and biosafety levels are illustrated table 15. See world health organization biosafety laboratory guidelines.
Orders can be placed with world health organization, who press, 1211 geneva 27. Health organization, laboratory biosafety manual, in its. Cdc 211112revised december 2009 unc school of public health laboratory safety levels u. Laboratory biosecurity concepts are introduced, and the latest regulations for the. The laboratory biosafety manual lbm3, first published in 2004, has provided guidance to laboratory workers on how to perform laboratory work safely, to laboratory managers on how to set up a managerial approach to biosafety and to regulatory authorities, to help them consider necessary aspects for the. For more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques. Emergency situations may include incidents or accidents, medical emergencies, fire, spills e. In the past year, the world health organization who reported sporadic human cases of h5n1 in cambodia, vietnam, indonesia, egypt and bangladesh. Laboratory guidelines research institute for tropical. Who published the first edition of the laboratory biosafety manual in 1983. This manual describes the minimum biosafety measures that should be implemented at the different levels of tuberculosis tb testing laboratories to reduce the risk of a laboratoryacquired infection. Guidance on regulations for the transport of infectious substances.
Department of health and human services, public health servicecentersfor diseasecontrol and prevention national institutesof health, hhspublication no. The manual now covers risk assessment and safe use of recombinant dna technology. The manual encourages countries to prepare specific. Canadian biosafety handbook, second edition canada. The classification of biological agents is a basic requirement for both biosafety and biodefense. Chapter 1620 canadian biosafety handbook, second edition. Biosafety and biosecurity center for global health. Who extended biosafety advisory group bag meetings, geneva, 2425 november 2014, and 15 december 2016 key recommendation 2014 revision to the who biosafety manual is both a necessary and a priority key recommendation 2016 general agreement for the proposed modifications to the manual, the revision of which remains a priority.
It is reported that 390 million people are infected annually, and about 96 million people among them. Laboratory biosafety is the process of applying a combination of administrative controls, containment principles, practices and procedures, safety equipment, emergency preparedness, and facilities to enable laboratory staff to work safely with potentially infectious microorganisms. The manual encouraged countries to accept and implement basic concepts in biological safety and to develop. Who laboratory strengthening tools world health organization. Interim laboratory biosafety guidance for extensively drugresistant xdr mycobacterium tuberculosis strains. The world health organization who reports that about 3. Biosafety and biosecurity in european containment level 3. Preventing transmission of infectious agents in healthcare settings pdf icon.
Many people assume that biosafety levels and biological risk groups are synonymous, when in fact there are many factors to consider when designating a biosafety level for a biological agent. There are four risk group classifications established in the nih guidelines and the world health organizations laboratory biosafety manual. World health organization laboratory biosafety manual, third edition this manual covers risk assessments, the safe use of recombinant dna technology, and guidelines for the commissioning and certification of. Recombinant dna molecules, and the world health organizations laboratory biosafety manual third edition. It is critical that all containment zones address situations where biosafety or biosecurity issues may arise as a result of an emergency. World organization for animal health oie guidelines for responsible conduct in veterinary research identifying, assessing, and managing dual use ivbw veterinary biosafety handbook. Further, among the provisions in the area of shared responsibility in biosafety and biosecurity is a provision for education and training that other internationally mandated regulatory regimes such. Good microbiological technique and appropriate use of biosafety equipment by welltrained staff remain the fundamental elements of laboratory biosafety. The quality of laboratory services thus not only directly affects the quality of health care for individual patients it is also crucial to a.
This manual is available in portuguese, french, spanish and english. As mentioned in the world health organization who laboratory biosafety manual, laboratory biosafety is the term used to describe the containment principles, technologies, and practices that are implemented to prevent unintentional exposure to pathogens and toxins or their accidental release. Safe laboratory techniques biosafety and recombinant dna technology. World health organization biosafety laboratory guildlines. General information on laboratory biosafety guidelines, see the who laboratory biosafety manual, 3rd edition 8. Wcln resources wisconsin state laboratory of hygiene. Who laboratory manual for the examination of human semen and spermcervical mucus interaction. Laboratory biosafety manual third edition, 2004 pdf, 1. Recent sporadic human cases in egypt, bangladesh, cambodia, vietnam and china indicate that appearance of other cases are expected and will likely occur in the future. Laboratory guidelines research institute for tropical medicine. Gli stepwise approach towards tb laboratory accreditation. Laboratory biosafety manual world health organization.
Who laboratory manual for the examination and processing of human semen op. Laboratory biosafety manual third edition is a wellwritten very well organized volume that provides the reader with a clear understanding of biosafety in the laboratory. Footnote 5 it is a guidance document for canadian facilities where human and terrestrial animal pathogens are handled, that updates the guidelines originally published as part ii of the. Stop tb partnership global laboratory initiative gli. Laboratory biosafety is fundamental to controlling exposure to pathogens, protecting the laboratory workforce and the wider community against inadvertent exposures or releases. Laboratory biosafety manual by world health organization.
Manual for the laboratory diagnosis of measles and rubella virus infection, 3rd edition, june 2018 from the world health organization who this manual provides information about the who laboratory global network for measles and rubella including detailed protocols for collection and handling of specimens for confirmation of measles and rubella. Department of agriculture usda and centers for disease control and prevention cdc tuberculosis laboratory guidelines centers for disease control and prevention. Major revisions to the 2014 institutional laboratory. Refer to the who laboratory biosafety manual 3rd editionii for appropriate biosafety practices. Who laboratory biosafety manual third edition world health. Who laboratory biosafety manual world health organization.
Who laboratory manual for the examination and processing of human semen 5th ed. Jun 27, 2014 biosafety in microbiological and biomedical laboratories, 5th edition, u. The manual now covers risk assessment and safe use of recombinant dna. Biosafety in microbiological and biomedical laboratories, 5th edition, u.
For more than 20 years, since it was first published in 1983 the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. The manual now covers risk assessment and safe use of recombinant dna technology, and provides guidelines for the. Abstract this laboratory manual references best practices from the clinical and laboratory standards institute clsi, the foundation for innovative new diagnostics find mgit procedure manual, and laboratory procedures from the national institutes of health tb research unit, as well as biosafety recommendations from the world health organization who and the us centers for disease control. Good microbiological technique and appropriate use of biosafety equipment by well trained staff remain the fundamental elements of laboratory biosafety.
The manual encouraged countries to accept and implement basic concepts in biological safety and to. Tuberculosis laboratory biosafety manual ncbi bookshelf. Investigations have shown that there are hundreds of unreported biosafety accidents, with laboratories selfpolicing the handling of biohazardous materials and lack of reporting. We compared and analyzed the biological agent laboratory biosafety category list and the defining criteria according to the world health organization who, the. Who guidance on regulations for the transport of infectious substances, 20 2014 whohsegcr2012. It is recommended that the following specimens be collected for the diagnosis of evd. There is still limited information on the risk posed by the reported coronavirus found in wuhan, but it would appear samples prepared for molecular testing could be handled as would samples of suspected human influenza 2, 79. Who laboratory biosafety manual having served for global biosafety community hereinafter, who manual for more than 20 30 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. Apr 20, 2018 laboratory biosafety is fundamental to controlling exposure to pathogens, protecting the laboratory workforce and the wider community against inadvertent exposures or releases. World health organization geneva 2004 laboratory biosafety manual third edition. For more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in.
Mycobacteriology laboratory manual centers for disease. Biological agents pose a serious threat to human health, economic development, social stability and even national security. Reliable and timely laboratory investigation results are fundamental to decisionmaking in almost all aspects of health services. Laboratoryacquired dengue virus infection by needlestick. World health assembly executive board accountability. Who laboratory biosafety manual, 3rd pdf icon external icon who laboratory biosafety guidance related to the novel coronavirus 2019ncov world health organization pdf icon external icon cdc 2007 guideline for isolation precautions. The manual provides university safety guidelines for those working with biohazards. References and sources for standard operating procedure and. Who library cataloguinginpublication data world health organization. Look on the internet page of the world health organization.
Apr 07, 2016 the world health organization who reports that about 3. Laboratory quality standards and their implementation. This handbook is intended to provide a comprehensive reference for all stakeholders in health laboratory processes, from management to administration to benchwork laboratorians. The maximum containment laboratory biosafety level 4 6. Since the occurrence of large epizootics of the avian influenza a h5n1 virus in southeast asia in 2004 and 2005, the virus continued to spread among avian populations geographically. Biosafety, transport and related legislation concerning. The canadian biosafety handbook cbh, 2 nd edition, 2016 is intended as a companion document to the canadian biosafety standard cbs, 2 nd edition, 2015. Biosafety and biosecurity center for global health science. Essential biosafety measures for tb laboratories 3. Risk group 2 agents that are associated with human disease which is rarely serious and for which preventive or. World health organization laboratory biosafety manual, third edition this manual covers risk assessments, the safe use of recombinant dna technology, and guidelines for the commissioning and certification of laboratories.
The world health organization who has long recognized that safety and, in particular, biological safety are important international issues. Guidelines for laboratoryfacility certification part ii. This biosafety manual is applicable to all laboratory, research, teaching, service, and support activities that may involveexposure to biohazard agents or materials ous or otherwisecomes under the purview of the institutional biosafety committee ibc at the university of montana. Who laboratory manual for the examination and processing. Guidance documents manuals ebsa european biosafety.
1127 187 1325 549 385 982 687 798 385 1264 1577 752 853 889 211 1229 1128 678 1243 948 420 1491 492 524 380 784 1386 206 558 2 564 206 550 1280 870 1487 244 692 935 1234 548